Protocol to maintain single functional mouse hematopoietic stem cells in vitro without cell division
Summary: This protocol details the isolation and in angry beavers shirt vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche.The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays.Following hibernation culture, HSCs can be used to study quiesce